Active Air Sampling Sop

6 1 6 place the cassette in the air sampler under an laf.

Active air sampling sop.

Analyse the microbial quality of air by sampling 100 litre of air at each of the locations using active air sampler. Passive air sampling passive air sampling i e settle plates is a frequently. Standard operating procedures for the passive air sampling 2 2 pas assembling the pas should be assembled from the bottom to the top. Active there are two primary methods for microbial air sampling.

Analyze the microbial quality of air by sampling 100 litres of air at each of the locations mentioned in annexure i using active air sampler carry the pre incubated scda plates and sterile ss head in a ss container to the sampling location. 1 impingers impingers use a liquid medium for particle collection. When the pre set sampling cycle is completed the plate is removed and incubated. 6 2 1 push the on off button.

Perform the growth promotion test of each lot of media used for environmental monitoring as per sop. The organisms are then visible to the naked eyes and can be counted for an assessment of the level of contamination. In active monitoring a microbial air sampler is used to force air into or onto its collection medium e g petri dish with nutrient agar based test media over a specified period of time. Place the axis in vertical position.

This nut will be the bottom limit. Fertility test of media. Carry the preincubated scda plates and sterile ss head in a ss container to the sampling location. 5 9 5 regulate the pressure of the air so that the flow rate is in between 140l to 180l per minute.

The following steps describe the way to assemble the passive air sampler. 5 9 6 ensure that m air t media cassette is in place before sampling is started. This leaves determination of the number ofsites for passive air sampling and surface sampling. Sterilise the stainless steel aspirating head of the air sampler prior to use by autoclaving as per sop.

5 2 operation of air. Active and passive monitoring. 6 2 2 after pressing the on off button the previously selected air sample volume retained in the memory appears on the display. Typically sampled air is drawn by a suction pump through a narrow inlet tube into a small.

Active air sampling requires the use of a microbiology air sampler to physically draw a known volume of air over or through a particle collection device and there are two main types. Active air sampling sites should be done at the same locations or as close as practical to avoid compro mising the other measure or the product integrity. Active sampling involves the use of an air sampling pump to actively pull air through a collection device such as a filter. The collected culture can then be incubated and.

Passive sampling however does not require active air movement from a pump. 5 9 7 close the media cassettes properly after sampling 5 9 8 clean the pump with 70 ipa before taking it to the next sampling location port. Enter into the aseptic area following aseptic area entry and exit sop of the concerned block.